Bio-click chemistry: Enzymatic functionalization of PEGylated capsules for targeting applications

MKM Leung; CE Hagemeyer; APR Johnston; C Gonzales; MMJ Kamphuis; K Ardipradja; GK Such; K Peter; F Caruso

Journal article

Bio-click chemistry: Enzymatic functionalization of PEGylated capsules for targeting applications

MKM Leung, CE Hagemeyer, APR Johnston, C Gonzales, MMJ Kamphuis, K Ardipradja, GK Such, K Peter, F Caruso

Angewandte Chemie International Edition | Published : 2012

DOI: 10.1002/anie.201203612

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Abstract

All sorted: The enzyme Sortase A was used to catalyze functionalization of PEGylated capsules with an activation-specific anti-platelet single-chain antibody (scFv). This enzymatic method allows fast, covalent, and site-directed functionalization of delivery vehicles under mild conditions. Activation-specific anti-platelet scFv-coated PEGylated capsules exhibited a high level of selective binding to thrombi, thus suggesting their potential for thrombosis therapy. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

University of Melbourne Researchers

Georgina Such Author

Karlheinz Peter Author

Frank Caruso Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

The authors are supported by the Australian Research Council by an Australian Postgraduate Award (M. K. M. L.), Discovery Projects (A.P.R.J., G. K. S., F. C.), a Future Fellowship (A.P.R.J., K. P.), and a Federation Fellowship (F. C.). Financial support was also provided by the National Health and Medical Research Council under the Program Grants 472665 (K. P.), 487922 (F. C.) and 1017670 (K. P.) and Career Development Award (C. E. H.), and in part by the Victorian Government's Operational Infrastructure Support Program.